Summary: Dendritic spine enlargement by synaptic activation is thought to increase synaptic efficacy underlying laguna 3hp dust collector learning and memory.This process requires forces generated by actin polymerization and actin-adhesion coupling (clutch coupling).Here, we describe a protocol to monitor actin filament retrograde flow and actin po
Nuclei Detection and Classification System Based On Speeded Up Robust Feature (SURF)
Tumors contain a high degree of cellular heterogeneity.Various type of cells infiltrate the organs rapidly due to uncontrollable cell division and the evolution of those cells.The heterogeneous cell type and its quantity in infiltrated organs determine the level maglinancy of the tumor.Therefore, the analysis of those cells through their nuclei is
Formaldehyde Fixation of Extracellular Matrix Protein Layers for Enhanced Primary Cell Growth
Coating tissue culture vessels with the components of the extracellular matrix such as fibronectin and collagens provides a more natural environment for primary cells in vitro and stimulates their proliferation.However, laguna 3hp dust collector the effects of such protein layers are usually rather modest, which might be explained by the loss immob